Biophysical and Biochemical Parameters of Organism During Experimental Malignant Tumor Growth

Ketevan Gambashidze, Nino Kipiani, Maka Topuridze

Have been studied biophysical parameters of blood, liver, spleen and cancer tissue by the method of Electronic-Paramagnetic-Resonance (EPR), activity of antioxidant enzyme catalase by the method of H. Aebi and ceruloplasmin concentration in the blood serum by the method of N. Babenko in white rats with Sarcoma C-45. Investigations revealed, that during Sarcoma C-45 growth antioxidant properties of blood are reduced, mitochondrial superoxiddismutase of tissues is inactivated, production of Mn2+, NO, xantinoxidase, cytochrom P-450 and peroxidation of lipids (POL) are activated, membrane structures' destruction, hemolysis and hypoxia are developed. After treatment with Plaferon LB, mentioned disturbances are vividly improved. It is stated, that administration of Plaferon LB decreases POL, restores antioxidant ability of organism, protects membrane structures, reduces hypoxia. Plaferon LB reveals features of antioxidant and NO modulator, due to which could be explained marked improvement of above-mentioned disturbances.

Keywords:   cancer, lipid peroxidation, EPR centers, blood, liver, spleen, catalase, ceruloplasmin, plaferon