The Methods of Biotechnology in the Vascular Transplantology

Igor Mikadze*, George Abzianidze*, Lela Kalandarishvili** 

To study the growth factor's influence on the vascular prosthesis and arterial pitch healing we used PTFE ("GORE", "IMPRA"), flourolavsan prostheses (Russia), xenoprostheses and tissue growth factor (GF) we have obtained in our laboratory. The endothelial cells (EC) were cultured under typical condition (1). Bioprostheses endothelized by autocells were implanted in dogs infrarenal aorta (78). The morphological investigation of functioning endothelized xenoprostheses showed that endothelialization of vascular substitution is a method of stimulating early healing of graft and provides forming of athrombogenal EC monolayer in the first days after implantation. The treatment of prostheses by GF was made by the original method. Experimental animals were divided into 4 groups depending on increasing GF concentration on the prosthesis wall: 0.05 mg/cm 2 of prosthesis - 0.00025 mg/cm² of prosthesis. There were made operations of grafting the carotids (54), femoral arteries (48), infrarenal part of abdominal aorta (174), torakoabdominal shunting (16). The best results were obtained in the third and the fourth groups. In 4 - 6 weeks after implantation all experimental prostheses and pitches had good cellular organization of neointima and endothelial coverage of luminal surface that was kept for the 6 months of observation.

Keywords: growth factors, vascular prosthesis, healing, neoangiogenesis, endothelial cells