The Antitoxic Effect of Plaferon LB on Pesticides in Tissue Culture Model

Lia Dzotsenidze, Jufar Mkervalishvili, Ruslan Gogiashvili, Vladimir Bakhutashvili

The antitoxic action of the preparation plaferon LB in the co inoculating cell lines of human and animal origin has been studied. Phosphoorganic compounds - pesticides, phosphamide, decis, and paarlan were used as toxic substances. In the indicated cells, pesticides caused (induced) cytotoxic, morphological and mutagenetic changes which degree depended on the kind of the toxins and cells being used. Mice (C-1300) neuro blastoma cells proved to be the most sensitive. A comparatively low sensibility was shown by pig embryo cells in which considerable morphological and cytogenetic changes were observed 48 hr after pesticides contact, especially under the action of paarlan. Preliminary processing of the cells with the preparation plaferon LB caused their partial protection from the cytotoxic and mutagenetic action of the pesticides used. On the basis of the results we have obtained, it's possible to suppose that the usage of plaferon LB for the prophylactic, as well as therapeutic purposes will considerably relieve consequences of pesticide-caused intoxication.

Keywords:  cell culture, plaferon LB, pesticides, toxin, morphological, mutagenetic, cytopathogenetic effects