The Influence of Mechanic Jaundice on Liver Regeneration after Partial Hepatectomy

George Megrelishvili, Diana Dzidziguri, Dimitri Kordzaya, Ekaterine Bakuradze

The present study was aimed to detect the molecular mechanisms of early response genes expression inactivation under influence of cholestasis in hepatocytes stimulated towards proliferation. Has been revealed, that occlusion of common bile duct affects the expression of early response genes in hepatocytes stimulated towards proliferation. Suppressive effect of cholestasis upon liver regeneration is realized only at the level of expression of cell proliferation controlling genes. Corticosterone (signal molecule) causes suppression of expression of early response genes controlling proliferation. After adrenal gland extirpation, the proliferative stimuli result in acceleration of mentioned gene expression in hepatocytes. Increased concentration of bile acid, as a result of cholestasis, causes inactivation of 11 β-hydroxysteroid dehydrogenase, providing increase in corticosterone concentration. In resected liver hepatocytes, due to corticosterone, early response gene partial suppression occurs, and processes related to reparative regeneration is delayed correspondingly.

Keywords:  cholestasis, early gene expression, liver regeneration, partial hepatectomy