The Malignant Tumor Growth and Reactive Forms of Oxygen and Nitrogen

Nino Bezhitashvili, Vakhtang Kipiani, Ketevan Gambashidze, Tamar Sanikidze, Natalia Pavliashvili

The purpose of this study was investigation of lipid peroxidation and nitric oxide (NO) concentration in blood and cancer tissue (in center and periphery) during sarcoma C-45 growth with the use of Electronic-paramagnetic-resonance method (EPR). Experiments have been carried out on 30 rats of mixed population (body mass 120-150 g). For detection of free NO concentration in blood, the spin-traps - Na diethyl-dithio-carbamate was used. The received data were analyzed statistically using the Student's t criterion. Have been stated, that in the process of sarcoma C-45 growth in central and particularly in peripheral region of the cancer tissue the NO synthesis is exaggerated that probably results in dilatation of supplying blood vessels in cancer tissue increasing trophicity and activating processes of proliferation. At the late period of cancer growth NO synthesis in central region of cancer decreases that could be explained by conversion of NO into peroxinitrite causing cell destruction. Along with that, lipid peroxidation in cancer tissue and organism is activated, which is manifested by increased intensity of oxidized ceruloplasmin, Met-Hemoglobinemia and increased concentration of Mn2+ and Fe2+ ions.

Keywords:  sarcoma C-45, blood, cancer tissue, lipid peroxidation, met-Hb, ceruloplasmin, nitric oxide