Taking the Samples for Isolation of Markers of Anaerobic Microflora and their Transportation in Case of Rapidly Progressive Periodontitis

Khatia Gogishvili, Klara Khetsuriani, Marina Mamaladze 

Department of Preventive Dentistry, Tbilisi State Medical University;
Department of Molecular Microbiology, Scientific-Research Institute of Experimental and Clinical Medicine, Georgia

Actinobacillis actinomycetecomitans, Porphyromonas gingivalis, Prevotela intermedia, Bacteroides forsythys and Treponema denticola, belonging to anaerobic microflora, are accepted as specific markers at rapidly progressive parodontitis. Their separation and identification are related with various technical problems. Noteworthy, that in the first hours of anaerobes growth, the above-mentioned microorganisms are especially susceptible to oxygen. Even miserable quantity of oxygen has killing effect on microorganisms. The aim of our work was creation of conditions for taking and transport of materials, providing absolute isolation of oxygen and thereby preventing contact of sample and oxygen. Materials for investigation has been taken and placed in Bukhner's vessel by insulin's syringe. Immediately after placing of material vaseline oil was added and like that the sample was delivered to the microbiological laboratory for farther investigations. For microorganisms' differentiation, along with Treponema denticola, the potato bullion was used and for identification - microscopy.

Keywords:  periodontitis, anaerobic microflora, markers, sample taking techniques