Influence of plaferon LB on LPS stimulated free radicals’ generation in Jurkat cell culture

Marina Nasrashvili, Ivane Datunashvili, Tamar Mchedlishvili, Manana Nasrashvili,
Tamar Sanikidze

Institute of Medical Biotecnology, Georgian Academy of Science, Tbilisi, Georgia

The free radical NO has many physiological functions. It has been shown that NO reacts with the cellular components, switches them from physiological to a pathophysiological stages and by this way involves in many diseases. From the above-mentioned the development of new drugs which selectively modulates NO activity is of great importance in modern medicine. We studied the influence of plaferon LB on LPS induced free radical generation in Jurkat cells culture. With EPR method we revealed basic EPR signal of spin-trapped NO in Jurkat cells culture. We show that stimulation by LPS promotes generation of nitric oxide, superoxide- and peroxyradikals in this culture. Decreasing of this free radicals EPR signals intensity by influence of plaferon LB indicates, that this preparation affects on NF-kB’ stability and by this way regulates proinflumatory genes expression and cells reactions on mitogens.

Keywords:  plaferon LB, free radicals, Jurkat cells