Nitric oxide and lipid free radicals at neonatal bilirubinemia

Tea Abzhandadze, Avtandil Kvezereli-Kopadze, Ether Japaridze, Tamar Sanikidze

I. Pagava Pediatric Scientific Research Institute, Tbilisi, Georgia

The present work was aimed to investigate intensity of free radical oxidation in the blood of neonates with hyperbilirubinemia. Has been defined content of lipoperoxides and free nitric oxide in the blood of practically healthy newborns and newborns with hyperbilirubinemia using the electronic-paramagnetic resonance method (EPR) and corresponding spin traps - α-phenyl-tert-buthylnitron (PBN) and Na-diethyldithiocarbamat (DETC) (SIGMA). Results of carried out investigations have shown that in the blood of neonates with hyperbilirubinemia process of lipid peroxidation is intensified leading to the activation of inducible NO-synthase expression and production of nitric oxide in organism.

Keywords:  nitric oxide, hyperbilirubinemia, neonates, free radicals, inducible NO-synthase