The changes in mitochondria respiration chain during NO-induced apoptosis and influence preparation plaferon LB in Jurkat cell culture

Merab Khizanishvili, Marina Nasrashvili, Maia Enukidze, Natia Janashia, Vano Datunasvili

Institute of Experimental Morphology, Georgian Academy of science, Georgia

The aim of our study was to establish the changes in mitochondria respiration chain during NO-induced apoptosis and influence preparation Plaferon LB in Jurkat cell culture. During incubation with natrium nitropruside (SNP) (24 and 48 hours) in Jurkat cells mitochondrial respiratory chain it was observed nitrosilation of electron transport proteins with formation of hemic (HbNO) and nonhemic (FeSNO) complexes, rising of mitochondrial respiration intensity, containing of free nitric oxide and intensification of superoxide- and lipoperoxide radicals generation. Under influence of Plaferon LB the concentration of nitric oxide and intensity of mitochondrial respiration in Jurkat cells restore, the intensity of oxidative stress decreased and revealed by reduction of superoxide and lipoperoxide radicals content.

Keywords:  mitochondria respiration chain, nitric oxide, apoptosis, plaferon LB, Jurkat cell